Neurofilament subunit (NFL) head domain phosphorylation regulates axonal transport of neurofilaments.
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Authors
Yates, Darran M
Manser, Catherine
De Vos, Kurt J
Shaw, Christopher E
McLoughlin, Declan M
Miller, Christopher C J
Issue Date
2009-01-14
Type
Journal Article
Research Support, Non-U.S. Gov't
Research Support, Non-U.S. Gov't
Language
en
Keywords
Alternative Title
Abstract
Neurofilaments are the intermediate filaments of neurons and are synthesised in neuronal cell bodies and then transported through axons. Neurofilament light chain (NFL) is a principal component of neurofilaments, and phosphorylation of NFL head domain is believed to regulate the assembly of neurofilaments. However, the role that NFL phosphorylation has on transport of neurofilaments is poorly understood. To address this issue, we monitored axonal transport of phosphorylation mutants of NFL. We mutated four known phosphorylation sites in NFL head domain to either preclude phosphorylation, or mimic permanent phosphorylation. Mutation to preclude phosphorylation had no effect on transport but mutation of three sites to mimic permanent phosphorylation inhibited transport. Mutation of all four sites together to mimic permanent phosphorylation proved especially potent at inhibiting transport and also disrupted neurofilament assembly. Our results suggest that NFL head domain phosphorylation is a regulator of neurofilament axonal transport.
Description
Citation
Yates, D. M., Manser, C., De Vos, K. J., Shaw, C. E., McLoughlin, D. M., & Miller, C. C. (2009). Neurofilament subunit (NFL) head domain phosphorylation regulates axonal transport of neurofilaments. European journal of cell biology, 88(4), 193–202. https://doi.org/10.1016/j.ejcb.2008.11.004
Publisher
License
Journal
European journal of cell biology
Volume
88
Issue
4
PubMed ID
ISSN
1618-1298