Electroconvulsive stimulation transiently enhances the permeability of the rat blood-brain barrier and induces astrocytic changes.
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Authors
Ito, Masanobu
Bolati, Kuerban
Kinjo, Tomoya
Ichimura, Koichiro
Furuta, Akiko
McLoughlin, Declan M
Suzuki, Toshihito
Arai, Heii
Issue Date
2016-11-30
Type
Journal Article
Research Support, Non-U.S. Gov't
Research Support, Non-U.S. Gov't
Language
en
Keywords
Astrocyte , BBB , Depression , ECT , Neurovascular unit , Tight junction
Alternative Title
Abstract
The blood-brain barrier (BBB) plays important roles in both the physiological and pharmacological state of the brain. Transiently enhancing the permeability of the BBB may allow use of more types of medications for neuropsychiatric diseases. Several studies have demonstrated that seizures cause a transient decrease in BBB integrity. We studied the timing of BBB changes following seizures and the role of astrocytes in this process. Rats received 10 applications of electroconvulsive stimulation (ECS). They were then infused with sodium fluorescein, a fluorescent substance that rarely passes the BBB, via the inferior vena cava. After 120min of circulation, the amount of sodium fluorescein in the brain was measured by two methods in vivo fluorescence imaging (total radiant efficiency) and the brain concentration of sodium fluorescein. To assess any changes to the BBB, we measured S100Β in serum, which is a standard marker of BBB breakdown that is expressed by astrocytes. We also examined ultrastructural changes following ECS. Total radiant efficiency and the brain concentration of sodium fluorescein were significantly increased in treated rats compared to controls when sodium fluorescein was injected immediately after ECS but not when the injection was performed more than 15 min after ECS. Astrocytic endfeet showed swelling around brain capillaries following ECS. In conclusion, ECS transiently enhances the permeability of the BBB, which may be accompanied by changes in astrocytic endfeet.
Description
Citation
Ito, M., Bolati, K., Kinjo, T., Ichimura, K., Furuta, A., McLoughlin, D. M., Suzuki, T., & Arai, H. (2017). Electroconvulsive stimulation transiently enhances the permeability of the rat blood-brain barrier and induces astrocytic changes. Brain research bulletin, 128, 92–97. https://doi.org/10.1016/j.brainresbull.2016.11.011
Publisher
License
Copyright © 2016 Elsevier Inc. All rights reserved.
Journal
Brain research bulletin
Volume
128
Issue
PubMed ID
ISSN
1873-2747